As we have shown before (Cai et al., 2007b), R1881 significantly increased the migration of LNCaP cells and this increased migration was completely abolished when Twist1 was siRNA depleted (Fig. 4F), suggesting that Twist1 mediates prostate cancer cell migration; there was no effect on cell proliferation (Fig. S6B). Here, TWIST1 is linked to prostate carcinoma.