We found that the inhibition of the RAGE/ERK/NF-κB axis significantly decreased the NETs or HMGB1 induced IL-8 production in glioma cells at both mRNA and protein levels (Figure 4F and G, Control vs. HMGB1: P = 2.26E-06, HMGB1 combined with FPS-ZM1 vs. HMGB1: P = 1.15E-05, HMGB1 combined with U0126 vs. HMGB1: P = 9.89E-05, HMGB1 combined with JSH-23 vs. HMGB1: P = 1.04E-04). Here, HMGB1 is linked to central nervous system cancer.