Increased expression of GRβ (which does not contain helix 12 of LBD but has a different amino acid sequence in helix 11 compared to GR-wt) was observed in hormone-insensitive autoimmune diseases (39), blood system diseases (40), cancer (41), etc. As shown in Figure 4 transfected GRβ exhibited little transactivation activity in the presence or absence of glucocorticoids, consistent with previous studies (39, 40). This evidence concerns the gene GZMB and autoimmune disease.