High expression levels of IL-6 and TNF-α were observed in the FO + NEC group at 24 h, 48 h and 72 h; however, the IL-6 levels in the FO + NEC group at 24, 48, and 72 h were lower than those in the NEC group by 4.30%, 9.40%, and 13.70%, respectively (P < 0.05), while the TNF-α levels in FO + NEC group were lower than those in the NEC group by 3.07%, 8.26%, and 11.37%, respectively. This evidence concerns the gene TNF and necrotizing enterocolitis.