In addition, we performed transwell (Fig. 5d), wound-healing (Fig. 5e) and 3D outgrowth invasion (Fig. 5f) assays to demonstrate that the phosphorylation of Ser26-USP51 is able to mediate the cell migration and invasion induced by CDK4/6 in breast cancer cells in vitro. This evidence concerns the gene USP51 and breast carcinoma.