This result is similar to a previous study suggesting that decreased expression of GSK3β is responsible for promoting the activity of Wnt/β-catenin signaling in HCC.43 Moreover, these findings suggested that HBX induced the MYH9/GSK3β/β-catenin/c-Jun-positive regulatory circuit to increase the activity of Wnt/β-catenin/c-Jun signaling and promote the CSC properties, migration, invasion, proliferation, and sorafenib resistance of HCC cells. This evidence concerns the gene GSK3B and hepatocellular carcinoma.