The activation of DNA-PKcs was associated with the development of severe ALD in wild-type (WT) mice treated with alcohol, with hepatocyte vacuolation, fibrosis, steatosis, increased lipid droplets, and elevation in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and AST/ALT ratio. The gene discussed is GPT; the disease is steatosis.