KCNJ13 and Leber congenital amaurosis: LCA is a congenital retinal dystrophy that causes significant vision loss at an early age.170 To verify that mutation in human KCNJ13 causes LCA, Zhong et al. employed CRISPR/Cas9 to create Kcnj13 mutant mice by zygote injection with sgRNA and spCas9 mRNA.