Subsequently, to validate our findings in another infection model, we repeated experiments using the cancer-derived iSLK.219 cell line stably infected with rKSHV.219, which constitutively expresses GFP under the control of the cellular EF1a promoter and RFP from the PAN promoter, an ORF50-responsive viral lytic promoter (42). This evidence concerns the gene ADA2 and infection.