TP53 and glioblastoma: This finding was replicated in a second model of GBM, in which NF1 and p53 were knocked-out in utero by injecting clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) constructs into the lateral ventricles of E14 mouse embryos, and electroporating to target progenitor cells in the SVZ.