The specificity of the designed capture probe was demonstrated by qPCR, analyzing the CEACAM5 expression (obtained by retrotranscription of RNA) in a non-metastatic CRC cell line (WIDR), in metastatic CRC cell-lines (T84 and LOVO), in a liver cancer cell-line (HEPG2), and in peripheral blood lymphocytes from healthy individuals. This evidence concerns the gene CEACAM5 and colorectal carcinoma.