To generate a vaccine that induces multi-antigen specific T cell responses, we designed an HBV immunogen encompassing the major HBV proteins (precore (PreC), core, polymerase (Pmut, with 8 point-mutations), preS1, preS2, and surface proteins), excluding the X-protein that has been associated with the development of HCC [20]. This evidence concerns the gene REG1A and hepatocellular carcinoma.