AIM2 and type 1 diabetes mellitus: To assess whether gut microbiota translocation is related to increased inflammation in the absence of AIM2, resulting in a higher risk of T1D development after the STZ injections, the WT and AIM2−/− mice were subjected to a daily antibiotic cocktail pretreatment (ABX: metronidazole, vancomycin, ampicillin, and neomycin) for 21 days, before the STZ injections, in order to deplete the gut microbiota.