Thus, we determined formation of the autophagosome-associated light chain 3-II (LC3-II), an indication of autophagy, from the cytosolic microtubule-associated protein LC3-I in SNU475 and Hep3B hepatoma cells, ectopically expressed with a green fluorescence protein tag of LC3 (GFP-LC3). This evidence concerns the gene MAP1LC3A and hepatocellular carcinoma.