Sanger sequencing identified a heterozygous variant of SLC12A2 (c.2941G>T), resulting in a D981Y amino acid change in I-4, II-3, II-4, III-1, and III-2, who had hearing loss, but not in the other subjects tested (Figs 1A and 2A, S2A Fig), indicating co-segregation of the variant with hearing loss, with the exception of I-3, who had hearing loss, but did not carry this variant. The gene discussed is SLC12A2; the disease is hearing loss disorder.