As depicted in Fig. 2, the three cell lines showed positive staining for CA 19-9, MUC1 and MUC16, with moderate to strong (2+ to 3+) intensity in more than 80% of tumor cells, with the exception of CA 19-9, which was only expressed in 20% of PUC-GBC2 cells. This evidence concerns the gene MUC16 and neoplasm.