Therefore, we analyzed the potential target miRNAs of NCK1-AS1 and the following genes via integrated online bioinformation system, glioma microarrays, dual luciferase reporter gene, RNA pull-down and RIP assays, after which we identified the interactions among NCK1-AS1, miR-138-2-3p and TRIM24. This evidence concerns the gene TRIM24 and central nervous system cancer.