Still, the NCK1-AS1 expression was increased in glioma cell lines U251, SHG-441, U87 and T98 compared to the normal glial cell line HEB, with the highest expression found in the U251 cell line while the lowest in the SHG-441 cell line (all p < 0.05) (Fig. 1e). Here, NCK1 is linked to central nervous system cancer.