Interestingly, HCC cells treated with both sFRP1 and Wnt16B displayed more apparent EMT, evidenced by the lowest level of E-cadherin and the highest levels of N-cadherin, vimentin, and snail when compared those treated with sFRP1 or Wnt16B alone (Fig. 3c), suggesting a synergistic interaction between sFRP1 and Wnt16B. This evidence concerns the gene VIM and hepatocellular carcinoma.