A total of 7 CRPs (JUN, FER, CTGF, TGFB1, STMN1, TMED2, and TMED10), which participate in “pathways in cancer” and are known to play potential regulatory roles were next selected for Western blotting (Fig. 3a) and immunofluorescence (Fig. 3b) validation, which confirmed the accuracy and robustness of our proteomics data. The gene discussed is TGFB1; the disease is cancer.