Firstly, although immunohistochemistry based on THPA and qRT-PCR based on urine samples confirmed the downregulation of CRYAB, ECM1, CGNL1, and GPX3 in HGBC and their diagnostic values, the exact molecular mechanisms of the these hub genes have not been investigated in the present study, and their prognostic values have not been proved by external validation. This evidence concerns the gene CRYAB and Hemoglobin C Measurement.