Similarly, PTEN was heavily mutated, with many nonsense and truncating mutations, as well as in-frame deletions, most of the mutations known to lead to inactivation of PTEN, and hot spot mutations were observed in the dual specificity phosphatase catalytic domain and the C2 domain of PTEN tumour-suppressor protein (Figure 8A and 8C). This evidence concerns the gene PTEN and neoplasm.