We, therefore, generated the PLSCR1- mut expression plasmid, expressed PLSCR1 and PLSCR1-mut that loses enzymatic activity in MCF7, T47D, and shPLSCR1-expressing MDA-MB231 and SUM159 cells, and examined the effect of PLSCR1 expression and enzymatic activity on breast cancer cell proliferation, migration and invasion. The gene discussed is PLSCR1; the disease is breast cancer.