To further confirm that PTEN, p18, p21, p57 and p63, which have been linked with cancer cell growth 38-42, are downstream targets of PRMT5, we designed shRNAs against PTEN, p18, p21, p57 and p63, and knocked down expression of these genes in PRMT5-depleted BGC823 cells. The gene discussed is CDKN1C; the disease is cancer.