For instance, specific SIRT1 deficiency in murine osteoclast (SIRT1flox/flox_LysM-Cre, the genetic approach used is consistent with our study) was found to activate osteoclastogenesis by promoting NF-κB signaling in vitro and results in osteoporosis in mice 30.The relationship between SIRT1 and miR-128 was tested using a pull-down assay, where a biotinylated SIRT1 probe was used to pull down SIRT1 mRNA. This evidence concerns the gene NFKB1 and osteoporosis.