Moreover, some studies have stated that the accuracy of serum ALT is relatively poor just at 40% for making the diagnosis of NAFLD.6 Due to the restrictions of serum transaminases as non-invasive biomarkers of NAFLD, irresistible evidences exhibited that definite cytokines which are derived of various biochemical events comprising of resistance to insulin, oxygen stress, apoptosis and inflammation play significant role in NAFLD progression. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.