Nearly 74% of participants (71 participants, 73.96%) knew that the serological diagnosis of toxoplasmosis was realized by ELISA based on the detection of IgG and IgM T. gondii antibodies, and only 14 (14.58%) of HPs knew that the avidity test can be applied as a complementary serology technique to date the primary infection during the first months of pregnancy (Table 2). Here, CD40LG is linked to infection.