To confirm the key role of HIF1α‐related glycolytic pathway in SETD1A‐induced GC cell proliferation, HIF1α special siRNA was used to knock down the expression of HIF1α in SETD1A‐overexpressing BGC‐823 and AGS cells, and then, CCK‐8 assay was performed to detect the GC cell proliferation. The gene discussed is SETD1A; the disease is gastric cancer.