Labeling of EVs with cell surface markers for the α-chain of the integrin receptor CR3 (CD11b) and the tetraspanin CD63 revealed an increase in the size of the populations of EVs produced in response to infection with wt A. fumigatus (antifungal EVs [afEVs]) relative to the size of the populations of spontaneously released EVs (sEVs) from uninfected cells (Fig. 1B to E; Fig. S1F and G and S2A and B). Here, CD63 is linked to infection.