Importantly, these experiments also demonstrated that PHATE_1-high tissues which do not permit stable EWSR1-FLI1 expression have (1) lower levels of R-loops compared to PHATE_1-low tissues and Ewing sarcoma (Figure 6 and Figure S8) and (2) low dependence on Fanconi Anemia and flap endonuclease genes (Figure 7 and Figure 8). Here, FLI1 is linked to Ewing sarcoma.