Mice with liver ERα-knockout (LKO) [38] and Gpr30-deficient mice (GPR30; orphan G protein-coupled receptor 30) [39] fed a HFD had increased liver triglycerides and diacylglycerides, and female rather than male mice had significantly lower HDL-C level along with an increase in fat liver accumulation with insulin resistance. This evidence concerns the gene ESR1 and Insulin resistance.