The targeting selectivity, extent of internalization, and killing efficacy of liposomes were evaluated in prostate cancer cells intrinsically expressing PSMA (human LNCaP and rat Mat-Lu cells) and in human umbilical vein endothelial cells induced to express PSMA (induced HUVEC), exposed to 225Ac-loaded liposomes (activity from 37 × 10−9 to 370 kBq/mL (10−9 to 101 mCi/mL). The gene discussed is FOLH1; the disease is prostate cancer.