As shown in Figure 4E, in HCT 116p53−/−, LQ1 treatment significantly increased the percentage of early apoptotic cells (Annexin V+ and PI+) from 1% in the untreated cells to 30% in treated cells, suggesting that apoptosis plays an important role in the antiproliferative effects of LQ1 on colon cancer cells. Here, ANXA5 is linked to colonic neoplasm.