Thus, pairwise Wilcoxon-rank sum tests were carried out to test staining differences between tissue types adjusting for multiple comparisons, the significant results of which are shown in Figure, 4B. As expected, HCC tissues had significantly higher ACSL4 staining than controls (P=5.9 × 10−9), CCAs (P=0.0057) and metastases to the liver (P=1.3 × 10−6). The gene discussed is ACSL4; the disease is hepatocellular carcinoma.