ACSL3 and cholangiocarcinoma: A box-whisker plot presenting ACSL3 immunohistochemical staining values for control tissues, HCC, CCA and metastases is presented in Figure 4A. A Kruskal–Wallis H test (one-way ANOVA) showed that there was a statistically significant difference in staining values between tissue types, χ2 (3) = 35.40, P<0.0005 (mean rank staining was 60.0 for control tissue, 128.0 for HCCs, 134.5 for metastases to the liver and 67.10 for CCA).