Subsequent experiments investigated Napepld, Cnr2, Faah, Naaa, Mgll and Tnfa (included in view of the known effects of TNFα upon components of the NAE/MAG systems in PCa cells50) for cells in serum-free medium treated with IL-6 for 3 h (Fig. 5b) or in serum-containing medium treated for 3 h (Fig. 5c) or 24 h (Fig. 5d). This evidence concerns the gene TNF and posterior cortical atrophy.