In tubular cells, MSTN was upregulated ~3 fold (a 3.32 ± 1.56-fold increase over controls) in DN kidneys showing no interstitial infiltration, and it was upregulated ~8 fold (a 7.89 ± 2.35 fold increase over controls) in the presence of tubulointerstitial infiltrates. Here, MSTN is linked to liver dysplastic nodule.