For example, in comparison with ccRCC, TFE3-RCC had higher proportions of very small and very large nuclei (see area_bin1, area_bin9, and area_bin10 in Fig. 3), which is in line with the fact that TFE3-RCC is more aggressive and associated with higher tumor grade30 because high-grade tumors have faster cell proliferation rate. This evidence concerns the gene TFE3 and renal cell carcinoma.