For example, female patients with lung squamous cell carcinoma (LUSC) had significantly higher levels of biomarkers, including CYT (p = 0.016; FDR = 0.099; Fig. 3b; Supplementary Fig. 6f), GEP (p = 0.0017; FDR = 0.038; Fig. 3b; Supplementary Fig. 6g), relative abundance of activated CD4+ T cells and activated CD8+ T cells (Fig. 3c), 20 out of 34 immune checkpoints (Fig. 3d) and TCR richness (p = 0.0028; FDR = 0.062; Supplementary Fig. 6h). The gene discussed is CD8A; the disease is squamous cell lung carcinoma.