To determine whether preexisting HMGN1 levels in TEXs would have any impact on the effect of additional N1ND on DC activation, we assayed TEXs, with a buoyant density of 1.13–1.23 g ml−1 (Supplementary Fig. 1a), from murine pancreatic cancer (Panc02), Lewis lung cancer (LLC1), lymphoma (EL4), breast cancer (4T1), HCC (Hepa1-6) cells, and DCs for HMGN1 (Fig. 1a). This evidence concerns the gene HMGN1 and hepatocellular carcinoma.