Correspondingly, persistent tumor inhibition and effector T cells infiltration into tumor sites were observed in DCTEX-N1ND-immunized C57BL/6 mice 4 weeks after tumor challenge with Hepa1-6 cells (5 × 105) injected subcutaneously as tumor volume and weight significantly decreased (Fig. 5g, h) and CD8+ effector T and TEM cells significantly increased in tumor tissues (Fig. 5i), and memory T cells in blood (Supplementary Fig. 5c, d) and the spleen (Supplementary Fig. 5e) significantly rose. This evidence concerns the gene CD8A and neoplasm.