To determine the effect of DCTEX-N1ND on the tumor microenvironment, we examined CD8+ T cells in blood and tumor tissues from day-21 orthotopic HCC mice treated with DCTEX-N1ND as DCTEX and HMGN1 were shown to function primarily via stimulation of CD8+ T cells and increased IFN-γ secretion13. This evidence concerns the gene CD8A and hepatocellular carcinoma.