To verify whether overexpression of miR-802 also impairs insulin transcription and secretion in vivo, we mimicked the obesity-associated increase in miR-802 expression in a transgenic mouse model by generating H11-CAG-LSL-miR-802 knock-in mice using CRISPR/Cas9 system (Supplementary Fig. 3e). Here, INS is linked to obesity due to melanocortin 4 receptor deficiency.