We also examined several genes implicated in NAFLD and/or associated with glucose and insulin metabolism such as Foxo1, Hsd11β1, Fgf21, G6pase, and Pepck; among these, only Foxo1 was upregulated in E4orf1-Tg mice (Fig. 6i). This evidence concerns the gene FGF21 and metabolic dysfunction-associated steatotic liver disease.