Importantly, retromer participates in the trafficking of lysosomal hydrolases (Cui et al., 2019; Seaman et al., 1997), and in the absence of Vps29, we documented accumulation of glucosylceramides along with ultrastructural evidence of brain lysosomal pathology similar to lysosomal storage disorders. This evidence concerns the gene VPS29 and lysosomal storage disease.