Moreover, we analyzed the co-expression of miR-186 and its targets in cancer tissues, and the data (collected from ENCORI database) showed an inverse correlation between miR-186 and PTEN, miR-186 and PIK3R3 in multiple cancer types, including in non-small-cell lung cancer, colon cancer, hepatic carcinoma, and breast cancer, and a positive correlation between miR-186 and APAF1 in several cancer types (Table 1). This evidence concerns the gene PIK3R3 and cancer.