Dual- luciferase reporter assay revealed that overexpression or knockdown of miR-194-5p respectively reduces or increases the fluorescence intensity in HEK293T cells expressing 3'-UTR of IGF1R or PPFIBP1 but not 3'UTR of IGF1R or PPF1BP1 which contains mutated miR-194-5p binding sites (Fig. 3a, b), meaning that miR-194-5p can directly bind to the 3'-UTR of IGF1R and PPFIBP1, and IGF1R and PPFIBP1 are targets of miR-194-5p in ovarian cancer. This evidence concerns the gene PPFIBP1 and ovarian cancer.