To determine whether cGAS directly contributes to CIN without the involvement of the immune system, to find a model suitable for our experimental setting we addressed the cGAS/STING levels in four cell lines: hTERT-RPE1, U2OS (human osteosarcoma cell line), HEK293T (human embryonic kidney cell line), and HeLa cells. This evidence concerns the gene STING1 and cervical squamous intraepithelial neoplasia.