Surprisingly, expression of Acaca was comparable between WT and Spp1−/− mice, while Srebf1, Fasn and Scd1 where even significantly downregulated in the Spp1−/− genotype (Figure 2A), indicating that the increased steatosis observed in Spp1−/− mice cannot be explained by enhanced de novo lipogenesis (dnl). Here, SPP1 is linked to steatosis.