To begin to identify the mechanisms and pathways disrupted by DUX4-fl expression in murine skeletal muscle, RNA-seq analysis was performed on gastrocnemius muscles isolated from control (ACTA1-MCM/+), FLExD/+ hemizygous, mild, moderate (MD9), and severe (SD9) bi-transgenic FSHD-like model mice (13 weeks old, n = 3 each), and analyzed for global mRNA expression levels (Table S1). Here, ACTA1 is linked to facioscapulohumeral muscular dystrophy.