Additionally, treatment with the ICI ipilimumab in combination with anti-TGF-β and anti-CD25 antibodies of syngeneic MPM in BALB/c mice resulted in (i) disease eradication in all treated animals, (ii) elevated levels of tumor-specific IgG antibodies in cured mice, (iii) failure to regrow tumors in cured mice when re-challenged with the same tumor, and (iv) response abolition in the absence of B-cells, suggesting that antibodies generated upon treatment contribute significantly to the curative effect [107]. This evidence concerns the gene TGFB1 and neoplasm.