To evaluate an anti-tumorigenic effect of SIRT1 in cancers harboring RAS mutations, we established doxycycline (Dox)-inducible HRASG12V oncogenic mutation cell lines by using a Tet-on system in control (Ctrl-iRas) or SIRT1-overexpressing (Sirt1-iRas) NIH3T3 cells, subsequently enriched by FACS sorting with fluorescence signals from humanized Kusabira-Orange protein (Figure S2A). This evidence concerns the gene SIRT1 and cancer.