in vivo, the mRNA levels of H2AX, ATM, ATR and CHK2 were remarkably up-regulated in the tumor tissues of mice following intragastric administration of genistein, compared with the tumor tissues of untreated mice (Figure 4I, P<0.05), indicating that long-term treatment has a significant effect on EsC cells in vivo. The gene discussed is H2AX; the disease is neoplasm.