It was observed that exposure to the NO donor S-nitroso-N-acetyl-penicillamine (SNAP) induced the expression and release of CGRP and other migraine-related factors, including substance P (SP), cholecystokinin (CCK), and prostaglandin E2 (PGE2), accompanied by the activation of glycogen synthase kinase 3β (GSK-3β) and the inactivation of PI3K/Akt. Here, TFF2 is linked to migraine disorder.